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Furthermore, studies found that LPS-induced macrophages in patients can release HMGB1 via extracellular vesicles (EVs), subsequently activating the NLRP3 inflammasome to trigger hepatocyte pyroptosis [46]

Moreover, the expression levels of genes were compared with those of the control group using Dunnetts test (GraphPad Prism version 9.5, GraphPad Software, La Jolla, CA)

Traditionally, in PCOS, one of the main aspects to consider is hyperglycemia, as it can trigger type 2 diabetes mellitus, cardiovascular diseases, the production of inflam-matory factors, and reactive oxygen species that cause oxidative stress and tissue damage 5

Flow cytometry and sorting For surface staining cells were incubated at 48 C, in the dark with fluorochrome-conjugated CD19, CD23, CD21-CD35 or CD35, IgD, IgM, CD1d, CD24, CD93, CD95, and GL-7 diluted 1:200 (refer to Supplementary Data 4 for details)